About the Event

Lipid nanoparticles (LNPs) are clinically successful nonviral vehicles for RNA cargo delivery, yet their interactions with biological systems remain incompletely understood. Upon exposure to biofluids such as pooled human plasma, proteins spontaneously coat LNPs, forming a protein corona that defines their biological identity.

In this webinar, Elizabeth Voke will present a quantitative, label-free proteomics workflow developed to characterize the LNP protein corona. You’ll learn how to isolate lipid nanoparticles and their associated proteins in a label-free manner using density-gradient ultracentrifugation with quality control checks. She will also explore how specific corona components impact the LNP delivery of RNA cargo, providing mechanistic insights into uptake and mRNA expression.

The findings, recently published in Nature Communications, reveal that specific proteins—such as vitronectin and C-reactive protein—can alter intracellular trafficking and mRNA expression.

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Webinar Objectives

1. Learn how to isolate lipid nanoparticles and their associated proteins in a label-free manner through density-gradient ultracentrifugation with quality control checks. 
2. Explore how specific corona components impact the LNP delivery of RNA cargo. 

Meet the Speaker

Elizabeth Voke, PhD
Postdoctoral Researcher
Landry Lab, University of California, Berkeley