Automated Solutions for ELISA

Your Guide to Workflow Intelligence

Enzyme-linked immunosorbent assays (ELISAs) are a common analytical tool for detecting the presence and concentration of a protein or other molecules of interest. Despite the ELISA method used, numerous reagent additions, wash steps, and incubation times make this workflow a labor-intensive endeavor with susceptibility to human error and variability in results. Automation of these steps relieves the labor burden with improved throughput and consistency.

Automated i-Series ELISA Solutions

Flexible and scalable to meet your growing needs

ELISA has been a common practice in the biology research laboratory since the 1970's. Despite its widespread for adoption in industry, its variability in results, susceptibility to human error, labor intensive workflow, and slow time-to-result remain hurdles for users looking to adopt ELISA in med to high throughput applications.

The Biomek automated workstation increases consistency of your ELISA method while increasing throughput. The flexibility of the i-Series workstations allows you to do as little or as much as you need, whether you’re doing a simple or a complex ELISA assay.

Label-Free ELISA Manual vs Automated

Label-free ELISA Manual vs AutomatedIntegrated: walk away, increased throughput

Your i-Series ELISA Workstation precisely prepares sample plates and processes each per your protocol, capturing all activities and data generated per sample and per well.

You are free to work on other tasks!

Direct Labeled ELISA Manual vs Automated

Direct Labeled ELISA Manual vs AutomatedIntegrated: walk away, increased throughput

Your i-Series ELISA Workstation precisely prepares sample plates and processes each per your protocol, capturing all activities and data generated per sample and per well.

You are free to work on other tasks!

Indirect Labeled ELISA Manual vs Automated

Integrated: walk away, increased throughput

Your i-Series ELISA Workstation precisely prepares sample plates and processes each per your protocol, capturing all activities and data generated per sample and per well.

You are free to work on other tasks!

Sandwich Labeled ELISA Manual vs Automated

Integrated: walk away, increased throughput

Your i-Series ELISA Workstation precisely prepares sample plates and processes each per your protocol, capturing all activities and data generated per sample and per well.

You are free to work on other tasks!

Competitive Labeled ELISA Manual vs Automated

Integrated: walk away, increased throughput

Your i-Series ELISA Workstation precisely prepares sample plates and processes each per your protocol, capturing all activities and data generated per sample and per well.

You are free to work on other tasks!

Workstation Solutions

Flexible and scalable to meet your growing needs

The Biomek automated workstations combine 30 years of system innovation with data centric software to deliver the confidence needed to walk away from the bench and allow your Biomek system to handle a variety of ELISA tests, managing repetitive steps associated with multiple dispensing, washing, and incubation steps.

We understand that each lab is unique. Depending on your workflow and requirements, we can provide an integrated solution that combines liquid handling automation with laboratory equipment of your choice to meet your specific ELISA applications.

i5 Label-Free ELISA

Despite ELISA’s widespread adoption in the industry, its variability and slow time-to-result remain the big hurdles for users looking to adopt ELISA in high throughput applications. The integration of the Octet system with the i-Series workstation offers an excellent alternative solution, enabling a label-free format whether you’re doing a simple assay or complex screening. OUR SOLUTIONS:

Biomek Automated Workstation

  • Continuous preparation of the standards and controls: precise and consistent dilution to establish an accurate standard curve.
  • On-time processing of the plates to and from your direct ELISA analyzer and incubator to ensure timely processing and reading while improving capacity.
  • Contamination-controlled environment with enclosure, HEPA filter*, & Biomek “Hold Labware” SW feature.

Biomek Suite of Software

  • SAMI EX: controls plate movements to and from integrated devices per scheduled time providing high consistency.
  • DART: links sample identification and assay results throughout the process

Integrated Direct ELISA Method such as Octet® Systems

  • Measure protein concentration and binding kinetics

Integrated Hotel

  • Increase throughput without need for intervention

Integrated Incubator

  • Consistent storage of the plates undergoing experimental assay

Automated Labware Positioners (ALPs)

  • Perform application specific tasks such as shaking or heating on the deck to expedite processing the plates

Biomek Validated Disposable Tips

  • Certified endotoxin-free and trace metal-free to eliminate contamination
  • Volume capacity: 30μl to 1070μl

i7 Label-Free ELISA

Despite ELISA’s widespread adoption in the industry, its variability and slow time-to-result remain the big hurdles for users looking to adopt ELISA in high throughput applications. The integration of the Octet system with the i-Series workstation offers an excellent alternative solution, enabling a label-free format whether you’re doing a simple assay or complex screening. OUR SOLUTIONS:

Biomek Automated Workstation

  • Continuous preparation of the standards and controls: precise and consistent dilution to establish an accurate standard curve.
  • On-time processing of the plates to and from your direct ELISA analyzer and incubator to ensure timely processing and reading while improving capacity.
  • Contamination-controlled environment with enclosure, HEPA filter*, & Biomek “Hold Labware” SW feature.

Biomek Suite of Software

  • SAMI EX: controls plate movements to and from integrated devices per scheduled time providing high consistency.
  • DART: links sample identification and assay results throughout the process

Integrated Direct ELISA Method such as Octet® Systems

  • Measure protein concentration and binding kinetics

Integrated Incubator

  • Consistent storage of the plates undergoing experimental assay

Automated Labware Positioners (ALPs)

  • Perform application specific tasks such as shaking or heating on the deck to expedite processing the plates
  • Increase throughput when space is limited for off-deck hotel (labware feeder)

Biomek Validated Disposable Tips

  • Certified endotoxin-free and trace metal-free to eliminate contamination
  • Volume capacity: 30μl to 1070μl

i5 Labeled ELISA

Optimal ELISA method depends on precise preparation of the plates, standards, controls, and samples through accurate and consistent pipetting, washing, incubation scheduling, and timely reading. The flexibility of the i-Series workstations allows you to do as little or as much as you need, whether you’re doing a simple or complex ELISA assay. OUR SOLUTIONS:

Biomek Automated Workstation

  • Preparation of the ELISA plates: addition of known antigen or antibody & blocking agent per set protocol, improving consistency and uniformity across all plates and wells.
  • Preparation of the standards and controls: precise dilution to reach the concentrations needed to establish an accurate standard curve.
  • Precise dispensing of samples and timely processing of the plates during incubation steps to ensure accuracy and consistency across all samples and plates.
  • Gentle handling through controlled speed, minimizing human error during washing steps.
  • On-time processing of the plates after stopping ELISA reaction through direct integration with a reader to ensure timely reading of your ELISA plates, while improving capacity for continuous production
  • Contamination-controlled environment with enclosure, HEPA filter, & Biomek “Hold Labware” SW feature.

Biomek Suite of Software

  • Biomek SW: on-time processing of each plate per your protocol reducing the chance for evaporation and/or light exposure of highly sensitive and expensive antibodies.
  • SAMI EX: controls plate movements to and from integrated devices per scheduled time providing high consistency. 
  • DART: links sample identification and assay results throughout the process

Integrated Hotel

  • Increase throughput without need for intervention

Integrated Incubator

  • Consistent storage of the plates undergoing experimental assay

Automated Labware Positioners (ALPs)

  • Perform application specific tasks such as shaking or heating on the deck to expedite processing the plates

Biomek Validated Disposable Tips

  • Certified endotoxin-free and trace metal-free to eliminate contamination
  • Volume capacity: 30μl to 1070μl

Integrated Reader such as SpectraMax i3X System

  • Timely reading of the ELISA plates

Integrated Plate Washer

  • Rapid addition and removal of wash buffers for ELISA

i7 Labeled ELISA

Optimal ELISA method depends on precise preparation of the plates, standards, controls, and samples through accurate and consistent pipetting, washing, incubation scheduling, and timely reading. The flexibility of the i-Series workstations allows you to do as little or as much as you need, whether you’re doing a simple assay or complex screen. OUR SOLUTIONS:

Biomek Automated Workstation

  • Preparation of the ELISA plates: addition of known antigen or antibody & blocking agent per set protocol, improving consistency and uniformity across all plates and wells.
  • Preparation of the standards and controls: precise dilution to reach the concentrations needed to establish an accurate standard curve.
  • Precise dispensing of samples and timely processing of the plates during incubation steps to ensure accuracy and consistency across all samples and plates.
  • Gentle handling through controlled speed, minimizing human error during washing steps.
  • On-time processing of the plates after stopping ELISA reaction through direct integration with a reader to ensure timely reading of your ELISA plates, while improving capacity for continuous production
  • Contamination-controlled environment with enclosure, HEPA filter, & Biomek “Hold Labware” SW feature.

Biomek Suite of Software

  • Biomek SW: on-time processing of each plate per your protocol reducing the chance for evaporation and/or light exposure of highly sensitive and expensive antibodies.
  • SAMI EX: controls plate movements to and from integrated devices per scheduled time providing high consistency. 
  • DART: links sample identification and assay results throughout the process

Integrated Incubator

  • Consistent storage of the plates undergoing experimental assay

Integrated Plate Washer

  • Rapid addition and removal of wash buffers for ELISA

Automated Labware Positioners (ALPs)

  • Perform application specific tasks such as shaking or heating on the deck to expedite processing the plates

Biomek Validated Disposable Tips

  • Certified endotoxin-free and trace metal-free to eliminate contamination
  • Volume capacity: 30μl to 1070μl

Integrated Reader such as SpectraMax i3X System

  • Timely reading of the ELISA plates

Integrated Centrifuge

  • Reliable and efficient sample preparation, ie separation of sera from whole blood

Biomek Automated Workstation

Improve throughput, consistency and uniformity across your ELISA method

  • Automate preparation of ELISA plates, standards and controls to ensure consistency and establish an accurate standard curve.
  • Increase precision through accurate dispensing of samples
  • Increase consistency through controlled and timely processing of the plates
  • Minimize human error during multiple washing steps
  • Improve efficiency by directly integrating required components
  • Simplify serial dilutions with built-in spanning transfer system
  • Improve analysis efficiency with sample and data tracking*
  • Take control of multi-day planning*
  • Monitor progress with platform status light
  • Remote system monitoring using on-deck cameras

*Possible through optional software packages DART and SAMI.

Suite of SW

Every time your sample moves, data moves. Biomek suite of software provides the most flexibility for modification, multi-day planning and data quality. Automatic data tracking and storage saves time and eliminatess manual data handling errors.

Few highlights include:

DART: Data Acquisition and Reporting Tool

  • Gather data and synthesis runtime information.
  • Generate data reports via MS Excel table and pivot views, and SQL views.
  • Integrate with your Laboratory Information Management System for automatic data transfer.

SAMI EX

  • Create schedules with the benefits of an optimized, predictable static schedule.
  • Save process time and control method variation with interleaving sub-processes.
  • Use live data to drive methods/processes.

SAMI Process Management

  • Use this calendar organizational tool to add, monitor, and plan for multiple methods and other events.
  • Schedule and run multiple processes simultaneously.
  • Integrate long-running processes into a centralized, visual workflow management tool.

Biomek Method Launcher

  • Select, set up, run, and track methods in a few simple mouse clicks.

DeckOptix Final Check

Address Setup Errors Before Your Run

DeckOptix Final Check - Address Setup Errors Before Your Run DeckOptix Final Check - Address Setup Errors Before Your Run

* Survey Data at Beckman Coulter Headquarter in Indianapolis

Biomek Validated Disposable Tips

  • Validated and approved for use on Biomek systems
  • Color-coded racks already in Biomek software for ease of use
  • Range of volume capacities from 30μl to 1070μl
  • Wide selection including:
  • Wide bore to prevent cell shearing
  • Filtered to prevent contamination
  • Conductive to enable liquid level sensing (span-8 pod)
  • Biomek pipette tips from Beckman Coulter are certified to be:
  • Endotoxin-free
  • Pyrogen-free
  • Trace metal-free
  • Sterile
  • Sterile filtered
  • RNase- and DNase-free

Bio-certification “free of” claims are defined as the lower limit of detection based on the sensitivity of the test method or instrumentation used.

Automated Labware Positioners (ALPs)

Workstation-integrated and interchangeable components that perform specific tasks. ALPs make it possible to configure the platform for accommodation of a wide range of applications.

Labware Feeder

  • Increase throughput when space is limited for an off-deck hotel and stackers

Shaking Peltier

  • Used for shaking or gentle mixing of microplates at speeds ranging from 100 rpm to 1400 rpm
  • Temperature control capable

Heating ALP

  • Controlled and gentle heating during incubation
  • Temperature control capable

Integrated Octet® Systems

Comprehensively characterize and optimize for cell clone lead(s), then, accurately measure target protein kinetics and concentration.

  • Label-free biophysical characterization for various stages of cell line development
    • Screen during clone selection to quickly rank by titer or off-rates
    • Reduce sample prep time by assaying proteins directly in crude mixtures
    • Measure specific binding interactions – determine affinity constants, epitope binning
  • Complete solution for label-free protein kinetics and quantitation
    • Ligand capture surfaces include antibodies, recombinant proteins, DNA, RNA, and virus-like particles
    • ELISA replacement offering increased productivity and accuracy, with off-the-shelf kits for Host Cell Protein and Residual Protein A impurity detection

Integrated SpectraMax® i3x Multi-Mode Microplate Reader

From imaging of cell confluence and viability under different treatment conditions to quantitation of nucleic acids and protein to western blot analysis, a wealth of new knowledge is captured using a single instrument.

  • Base model includes absorbance, fluorescence, and luminescence read modes for 6- to 384-well microplates
  • User-exchangeable detection modules expand the system’s detection capabilities
  • Engineered for performance with Spectral Fusion™ illumination for increased sensitivity across the entire excitation range
  • Cooled photomultiplier tube (PMT) for improved detection in low light.
  • The SpectraMax Injector Cartridge with SmartInject™ Technology allows for flash based applications, including dual luciferase and ATP assays.
  • The SpectraMax MiniMax 300 Imaging Cytometer option allows for live cell imaging and StainFree™ analysis

Beckman’s Integrated Solutions team have integrated 300+ third-party devices from 60+ manufacturers to meet your diverse and ever-evolving automation needs.

Examples of analyzers integrated:

Beckman’s Integrated Solutions team have integrated 300+ third-party devices from 60+ manufacturers to meet your diverse and ever-evolving automation needs.Beckman’s Integrated Solutions team have integrated 300+ third-party devices from 60+ manufacturers to meet your diverse and ever-evolving automation needs.

Biomek Automated Workstations are not intended or validated for use in the diagnosis of disease or other conditions. Beckman Coulter Life Sciences genomic reagent kits are for research USE only.

How Automation Helps to Improve ELISA

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