Echo Dose-Response Software

Improve Results with Direct Dilution

Prepare dose-response and standard curves using the unique ability of the Echo Liquid Handler to perform Direct Dilution. In a single protocol, the intuitive graphical user interface guides the transfer of samples controls and back-fill solutions for every curve. Reliable dose-response determination starts with liquid transfers with exceptional accuracy:

  • Visualize curve layouts, starting concentrations, final concentrations, and normalization requirements
  • Produce row- and column-wise dilutions
  • Replicate curves in adjacent wells, sequentially or across plates
  • Automate normalization
  • Import a pick list to identify samples for dilution
  • Errors do not compound as in serial dilutions

Direct Dilution Eliminates Carryover and Improves Results

Direct Dilution is a precise method for generating drug concentrations used in dose-response experiments. As opposed to serial dilution, nano- and microliter volumes are transferred in proportion to the desired concentration. With tip-based liquid handling, direct dilution is often not practical or possible. Dilution using the serial method can lead to inaccurate results due to error propagation.

Automation Software Echo Dose-Response Direct Dilution                    Automation Software Echo Dose-Response Potency Assays

Figure: Example of a 11-point curve created using Direct Dilution. The image to the left shows how the desired concentrations are created by transfers directly from the stock sample and two intermediate dilutions of the stock sample. The resulting IC50 is shown to the right.

Assemble dose-response curves by directly transferring different sub-microliter volumes from one or more stock concentrations to achieve the desired final concentration at each point. Acoustic, non-contact transfer with Echo Liquid Handlers eliminates tips, carryover, and dilution errors.

Echo Dose-Response Specifications

Description Specifications
Operating system

Microsoft® Windows 7 (32-bit or 64-bit), or Microsoft® Windows 10 64-bit (Windows

7 compatibility mode); Windows.Net Framework 4.62 or greater

CPU Intel® Core™ i5 or later
Memory 4 GB or greater
Network connection 10/100/1000 BaseT (2 network connections are required) 
Network protocol TCP/IP
Hard drive 4 GB Free Space Available
Video resolution 1920 x 1080 recommended resolution (1440 x 900 minimum resolution)
Echo Client Software 2.6 or later
Echo.Net Framework 1.7 or later